FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Biodegradation, fertilizer, hydrocarbon, lime, Mucor, nitrate
Bioremediation of spent lubricating oil contaminated soil by amending with 10% lime fertilizer was studied for a period of 56 days. Bacteria and fungi were enumerated using serial dilutions and pour plate method. The bacteria counts ranged from 1.0 x 103 to 7.6 x103cfu/g in unpolluted soil (UPS), 1.3 x 103- 8.8 x 103cfu/g in oil polluted soil (PS), 1.0 x 103 to 9.2 x 103cfu/g in lime amended polluted soil (AMD).Higher counts were observed in AMD than UPS and PS. The fungal counts ranged from 4.0 x 103 to 1.1 x 103 in UPS, 1.7 x 103 to 2.2 x 103¬¬¬ in PS, 1.0 x 103 to 1.9 x 103 in AMD. There was no significant difference in the bacteria and fungi counts at 5% probability level. The organisms isolated were Staphylococcus spp, Micrococcus spp Bacillus spp, Pseudomonas, Proteus spp, Penicillium spp, Mucor spp, Aspergillus spp and Neurospora spp. The pH (6.30±0.40), moisture (7.60±2.80), nitrate (1.95±1.60) and phosphorus (11.22±2.04) were higher in AMD than UPS and PS. The results of this study indicates that lime fertilizer can be employed for bioremediation of spent lubricating oil polluted soil by increasing the nitrate and phosphorus level of the soil which in turn support the growth of hydrocarbon-utilizing microorganisms.
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